Amelioration of Myogenin, Bcl-2 expression and DNA damages in myocytes of Trichinella spiralis-infected mice after immunization with gamma radiation-attenuated larvae

نویسندگان

  • E.N. Hafez Health Radiation Research, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
  • M.A.E.M. El-Kholy Al Azhar University, Faculty of Medicine, pathology Department, Cairo, Egypt
  • W.A.M.S. El-Kholy Al Azhar University, Faculty of Medicine, Parasitology Department, Cairo, Egypt
چکیده مقاله:

Background: In trichinosis, the presence of muscle larvae inside skeletal striated muscle causes loss of identity and function of myotube through the releasing of myogenin and myosin. Considering the dominant role of vaccination, research on gamma radiation-attenuated vaccine has very promising value. The goal of this study was to determine the efficacy of vaccination with radiation-attenuated Trichinella spiralis larvae on the subsequent infectivity. This goal was to be achieved by expression of myogenin, BcL-2 and DNA damages of myocytes in experimental animals. Materials and methods: Mice were randomly assigned to serve as normal control, infected control, vaccinated unchallenged and vaccinated challenged groups. Histopathological and immunohistochemical assessments of skeletal muscle tissues of diaphragm were conducted using different stains. Also, DNA damages of myocytes by comet assay were evaluated. Results: An improvement of histopathological changes and DNA damages of myocytes with significant decreased expression of myogenin and BcL-2 in vaccinated challenged group was detected. Conclusion: Vaccination with gamma radiation- attenuated larvae exerted protective effect against challenged infection. This may pave the way to new protective modality in trichinosis control.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Expression of MyoD and myogenin in muscles of mice experimentally infected with Trichinella spiralis or Trichinella pseudospiralis.

We developed a detection system for myogenic regulatory factors such as MyoD and myogenin. Adapting the method we performed a longitudinal analysis of such regulatory factors after infection with T. spiralis and T. pseudospiralis. MyoD and myogenin were expressed from the early phase of cystogenesis in T. spiralis infection. The expression returned to the normal level after 18 days from the inf...

متن کامل

Infectivity of Trichinella Spiralis Larvae

Time of survival and infectivity of Trichinella spiralis larvae in pig muscle tissue, buried at various depths in the ground were assessed. In the pork pieces the number of infective larvae was 250 ML/g. Meat originated from pig halves was divided in 39 equal pieces, 0.7 kg each, disposed in three groups of 1 3, and buried in depths of 30, 50, and 100 centimeters respectively. The pork was dug ...

متن کامل

Modulation of particle uptake in Trichinella spiralis-infected mice.

Mice were infected with 170 Trichinella spiralis larvae, and their blood clearance of carbon particles was studied 4, 7, 13, 32, and 135 days later and compared with that of noninfected control mice. Clearance in mice with a 4-day-old infection was comparable to that in the controls; clearances in mice with 7- and 13-day-old infections were increasingly accelerated and significantly different f...

متن کامل

Amelioration of influenza-induced pathology in mice by coinfection with Trichinella spiralis.

Illness due to respiratory virus infection is often induced by excessive infiltration of cells into pulmonary tissues, leading to airway occlusion. We show here that infection with Trichinella spiralis results in lower levels of tumor necrosis factor in bronchoalveolar lavage fluid and inhibits cellular recruitment into the airways of mice coinfected with influenza A virus. Infiltration of neut...

متن کامل

Reduction of muscle larvae burden in rats experimentally infected with Trichinella spiralis.

In Wistar rats infected with 500 to 2,500 Trichinella spiralis larvae the muscle larvae intensity (larvae per gram-l.p.g.) was measured from 20 to 180 day post infection (d.p.i). The l.p.g. increased to day 40-50 p.i. and decreased thereafter. The highest reduction took place between 60 and 120 d.p.i. with intermediate inoculum of T. spiralis larvae. The mechanism of the reduction of T. spirali...

متن کامل

Distribution of Trichinella spiralis larvae in selected muscles and organs of experimentally infected swine.

Thirty-two Hampshire-Yorkshire pigs (6 to 8 wk old) were inoculated with the Beltsville strain of Trichinella spiralis at a level of about 880 larvae/kg of body weight (about 15 kg). At about 100 kg, the pigs were slaughtered and 10-g samples of muscle and other tissues were digested in pepsin-HC1 and examined microscopically for T. spiralis larvae. The mean number of larvae recovered/gram was:...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 18  شماره 4

صفحات  699- 706

تاریخ انتشار 2020-10

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023